Microbiological culture remains the most sensitive method for detecting viable and infectious bacteria, but these methods often require at least 24 hours to visibly identify bacterial growth.

Here, we review the principles underpinning lateral flow assays and the historical evolution of theory to predict their performance.

To reduce the risk of these measurement errors, a new wider tape was developed.

A novel algorithm was developed to evaluate the sharpness of images captured with the MobileODT’s digital cervicography device (EVA System), in order to, eventually provide feedback to the health worker.

This framework is useful for stakeholders from test developers to clinicians to identify the trade-offs for diagnostic biomarkers for any use case.